Page 1 of 2: biochem---northern blotting

cma · Credits: 1011 My Scrapbook My Reading List 3 Books

hi anyone please tell nortern blotting requires1]amplificationof tRNA 2]amplication of rRNA3] amplication of nucleotides4] DNA[/img][/i]

skjeev · Credits: 8220 My Scrapbook

i think it should be mRNA?BUT no option given!

ujwals · Credits: 4860 My Scrapbook

#2???????????

THEROCK · Credits: 1456 My Scrapbook

yaah i think it should b mrna but if no other option then its 2

viladd · Credits: 163 My Scrapbook

The answer is 4.

Small stretch of DNA is synthesized to be used as a probe. Northern Blot does not involve amplification of any RNA at any step. This is crucial because many times, Northern Blot is used to compare the endogenous levels of an RNA in different tissues, or differents RNAs in the same tissues.

Good luck Medicos. Some of the answers on this forum seem very naive. I pity that medical students in India do not get any research experience.

jayanta · Credits: 34977 My Scrapbook My Reading List 45 Books

GOOD EXPLANATION.
REGARDS.

vivek_patel · Credits: 2336 My Scrapbook

option 4

bully · Credits: 2673 My Scrapbook

northern blot is used to find out size and quantify rna molecules.
correct me if im wrong

Subbernaut · Credits: 1067 My Scrapbook

Northern blot is used to analyse dna in a given saple using the array of dna available
Southern blot is used to detect dna in a given sample ,, usually a confirmatory test
Western blot is done ot detect a specific protein in a given sample ..

gynaedoc · Credits: 316 My Scrapbook

Please quote reference........as far as my knowledge goes.....northern blotting is for Rna and southern for DNA

		Sign In New User? Sign Up Sign in to access your control panel and messenger!
				TechZone \| SpiderNevi \| HowTo? \| Scrapbook!