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Quick Scroll addition of glucose to stored blood....why??? 07.08.04 (4 years ago) #1
addition of glucose to stored blood....why???
1.prevent hemolysis
2.provide nutrition
3.increases hemoglobin content]
4 increase 2-3 DPG
KIMDLY ANSWER WITH EXPLANATIONS
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Quick Scroll DPG 07.08.04 (4 years ago) #2
to increase DPG which carries O2
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Quick Scroll 07.08.04 (4 years ago) #3
Quote:
increase DPG which carries O2


Just to elaborate further-
As per the oxygen -Hb dissociation curve.Increase in concn of 2,3 DPG shifts the curve to the right.ie. will help in more of unloading of oxygen to the tissues. hence addition of glucose to the blood will produce more 2,3 DPG as a result of glycolysis & serve theis purpose. Yet as afar as possible, FRESH blood is the best choice for transfusion irrespective of whether u add dextrose or not, bcoz platelets & coagn factors r potent only if blood is fresh.
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Quick Scroll 07.08.04 (4 years ago) #4
Hi Mani,

i have a different opinion :

INOSITOL IS ADDED IN BLOOD TO INCREASE 2-3-DPG .
(ref. Lippincot-biochem)

glucose in stored blood provides nutrition.
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Quick Scroll 07.09.04 (4 years ago) #5
hi nadu... yeah that s what i was wondering ... so are u sure that glucose is for nutrition.... ???
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Quick Scroll 07.09.04 (4 years ago) #6
hey nadu!!!
still there is a confusion man .!!!
tell me do we want to decrease the 2-3 DPG in the stoed blood or do we want ti increase it...????
its been quoted ... thet acid cutrate dextrose is added to stored blood and this causes a preservational injury... that is it causes levels of 2-3 DPG to dcrease.
... so how can the glucose increase the 2-3 DPG level...????
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Quick Scroll 07.09.04 (4 years ago) #7
ya da i too believe tht it decreases 2,3 DPG and glucose just provide nutrirtion......but a tricky situation will again refer some text and try to find valid answer....
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Quick Scroll 07.09.04 (4 years ago) #8
Han yaar Mani,

Glucose for nutrition &

Inositol to increase 2-3-DPG

PLEASE REF. Lippincott 2nd edi.- Biochem.(in the topic of Hb)
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Quick Scroll 07.09.04 (4 years ago) #9
basically we want to increase 2-3 DPG or do we wanna devrease it in the stored blood??
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Quick Scroll 07.10.04 (4 years ago) #10
basically in stored blood level of 2-3-DPG DECRESES.......SO that will shift oxy. diss. curve to right........less oxy. is supplied due to less oxy. delivary.

therefore in order to "MAITAIN" the level of 2-3-DPG we add INOSITOL
which increases the depressed 2-3-DPG in stored blood to normal.

Yaar Mani tum ek bar Lippincott padh lo, everything will b clear !

its a exellent book for concepts of bio-chem !
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