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Nagging Q Forum Hot - Library - Unanswered
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ardySend an Instant Message to ardy  




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Quick Scroll Nagging patho/DNA 12.07.05 (2 years ago) #1
in situ dna nick end labelling can quantitate?
a)fraction of cells in apoptic pathway
b)fraction of cells in s phase
c)p53 gene product
d)bcr/abl gene
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Quick Scroll 12.07.05 (2 years ago) #2
I'd read somewhere its for quantitation of cells in apoptotic pathway.No explanations though.
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Quick Scroll 12.07.05 (2 years ago) #3
ans a)


an in situ method for detecting areas of DNA which are nicked during apoptosis; terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 primer OH ends of either single- or double-stranded DNA; assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA fragmentation


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Quick Scroll 12.07.05 (2 years ago) #4
thanx ,that was really clear.
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Quick Scroll 12.07.05 (2 years ago) #5
thanx ,that was really clear. icon_biggrin.gif
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