Page 1 of 1: CHROMOSOME WALKING

charan66 · Credits: 536 My Scrapbook

WHAT IS IT?

charan66 · Credits: 536 My Scrapbook

CLONIG EVERYTHING IN A GENOME AROUND A KNOWN PIECE OF DNA

akanksha · Credits: 41385 My Scrapbook

Chromosome walking is a procedure to find and sequence a gene whose approximate position in a chromosome is known.

For this we need a small genomic clone which is used as a probe.(a genomic clone is usually a small segment of DNA).

With this probe as a guide, we isolate 'new' clones containing this sequence and adjacent sequences encoding the next portion of the genome.

The end sequence of the second clone is used to isolate a third clone and so forth until a series of overlapping clones are isolated.

Hence at every step,we get a genomic clone which is longer than the first,though containing the initial similar sequence.

Hence,its literally walking along the length of the chromosome with every step larger than the first.

RxPG_Team · Credits: 19673346 My Scrapbook

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steady · Credits: 142 My Scrapbook

hi all,
there's also chromosome jumping.
while you can walk in steps of 30,kbps in chromosome walking, you can jump to even distantantly located genes using chromosome jumping.
however it involves restriction enzymes,ligation,etc. to skip the region between known points on the chromosome, usually 100kbps.

Guest · Credits: 84136 Preparing for: MRCOG Part 1 My Scrapbook

then how it is differant from linkage analysis

steady · Credits: 142 My Scrapbook

Linkage analysis is done for mapping the genes. In Chr.Jum, the position of the genes is known which are usually far apart from each other on the chromosome. Chr.Jum is used for making libraries of such genes of interest which are at a distance of =/> 100kbps fro each other. The genes are brought nearer to each other by producing sticky/blunt ends on either side of the genes, ligation if these ends-thus the genes are brought nearer. Like this:

------1---A=========================B---2---------

-----/1---A=========================B---2/-------

1---A=========================B---2

Ligate at positions 1 & 2:

========A---12---B=======

Remove unwanted DNA:

=A---12---B=

This is our insert.
Tranfect into a vector DNA and make clones-library is obtained.

anumeha_thaware · Credits: 621 Preparing for: DNB Part 1 My Scrapbook

hey guys can anyone please help me out.....

want some information regarding biotechnology.....like where's the course available and whats the scope and what are the qualifications for it??? it would be really helpful for me...

thanx....

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