Page 1 of 1: E-test

nadu · Credits: 17470 My Scrapbook

What is E-test ?

where it is done?

sorry i have no options !

shankardada · Credits: 1867 My Scrapbook

Mycobacterium tuberculosis strains resistant to streptomycin (SM), isoniazid (INH), and/or rifampin (RIF) as determined by the conventional Löwenstein-Jensen proportion method (LJPM) were compared with the E test, a minimum inhibitory concentration susceptibility method.

manipalguy · Credits: 16015 My Scrapbook

hi nadu.. i just got the following.. it seems E test is used for testing some antibiotics ...read the following....

Susceptibility testing for ESBL-producing organisms
The rising importance of ESBL-producing organisms has mandated effective screening methods for their detection. Use of aztreonam or ceftazidime resistance as a marker misses approximately 15-20% of ESBL-producing organisms. Resistance to cefpodoxime as a screening method, with sensitivity breakpoints of >2 mcg/mL by minimal inhibitory concentration or <22 mm by disk diffusion (for a 30-mcg cefpodoxime disk), has a sensitivity of at least [snip]% for ESBL detection.
Different tests that help confirm ESBL susceptibility are available. One test involves using disks that contain cefotaxime and ceftazidime alone and disks containing a combination of clavulanic acid with these antibiotics. These are placed on Mueller-Hinton agar. A positive test result is defined as a 5-mm or greater increase in the size of the zone diameter for either cefotaxime or ceftazidime tested in combination with clavulanic acid versus the zone for either antibiotic tested alone. Another method is the E-test screen, which evaluates third-generation cephalosporins with and without a beta-lactamase inhibitor. Finally, the Vitek ESBL test, which is an automated broth microdilution test, uses cefotaxime and ceftazidime alone and in combination with clavulanic acid.
A good screening strategy might include a cefpodoxime screen followed by confirmatory disk diffusion for screen-positive isolates. The Vitek test has sensitivity of at least 99.5% and specificity of 100%. It is a reliable single-test alternative.

Perform testing with antibiotics. Recommended methods include agar microbroth and macrobroth dilution. Newer methods include the E-test and the spiral gradient endpoint system. Test the following agents:

Penicillin

Broad-spectrum penicillin

Penicillin plus a beta-lactamase inhibitor

Clindamycin

Chloramphenicol

Second-generation cephalosporins such as cefoxitin

Newer quinolones

Metronidazole

Carbapenems

this Etest is used in testing antibiotics for organisms like,bacteroides,klebsiella,peptostreptococuus, and candidiasis.

nadu · Credits: 17470 My Scrapbook

thanx mani........its a really helpful.

manipalguy · Credits: 16015 My Scrapbook

at your service nadu!!!

		Sign In New User? Sign Up Sign in to access your control panel and messenger!
				TechZone \| SpiderNevi \| HowTo? \| Scrapbook!