Page 1 of 1: Microbiology: diagnosis of P. falciparum

cingulate_gyrus2006 · Credits: 22790 My Scrapbook

this is an AIIMS 2004 NOVques.
Which one of the following is detected by antigen detection test used for diagnosis of P. falciparum malaria.

a) Circum-sporozoite protein.
b) Merozoite surface protein.
c) Histidine – Rich- Protein I(HRP –1)
d) Histidine- Rich protein II(HRP – II)

drsmita_s · Credits: 18609 My Scrapbook

yup answer is indeed hrp 2 .
harri page 1225
these are rapid ,simple , sensitive and specific antibody based diagnositc tests which detect P. falciparum specific histidine rich protein 2 (hrp2)
requiring finger prick blood.

aap · Credits: 2289 My Scrapbook

hrpII is deteced by ag detection tests.the protein is present only in dividing or live parasites.

cingulate_gyrus2006 · Credits: 22790 My Scrapbook

It is an immunological test so it doesn’t depent weather it is dividing state or not.

Harrison says,,
PfHRP2-based tests may remain positive for several weeks after acute infection. This feature is a disadvantage in high-transmission areas where infections are frequent but is of value in the diagnosis of severe malaria in patients who have taken antimalarial drugs and cleared peripheral parasitemia (but in whom the PfHRP2 test remains strongly positive) or where malaria is alleged to have been eliminated..

So it can be positive even if there is no parasite in blood..(Treated)

vivek_patel · Credits: 2336 My Scrapbook

HRP 2

hariompriyom · Credits: 1885 My Scrapbook

[list=] IN CLINICAL PRACTIS IT IS USED AS ---PARACHECK PF -IS A RAPID SELF PERFORMING ,QUALITATIVE,TWO SITE SANDWITCH IMMUNOASSAY FOR THE DETECTION OF P.FALCIPARUM SPECIFIC HISTIDINE RICH PROTEIN-2 (HRP-2) IN WHOLE BLOOD SAMPLES. HRP-2 IS A WATER SOLUBLE PROTEN THAT IS RELEASED FROM PARASITISED ERYTHROCYTES OF INFECTED INDIVIDUALS & IS SPECIFIC TO THE P. FALCIPARUM SPECIES. IT USES THE PRINCIPLE OF IMMUNOCHROMATOGRAPHY.[/list]

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